INTRODUCTION OF DNA INTO CELLS
Biotech Glossary |
Bioinformatics |
Lab Protocol |
Notes |
Malaysia University |
2. Amplification of Baculovirus Plaques and Preparation of High Titer Virus Stocks
3. Baculovirus Transfection Using Calcium Chloride
4. Calcium Phosphate Transformation of Mammalian Cells
5. DNA Transfection of Eukaryotic Cells Using Calcium Phosphate
6. Drosophila Tissue Culture Cell Transfection
7. Electroporation of Competent E. coli TG1 Cells with Antibody Display Phagemid DNA
8. Electroporation of COS-7 Cells
9. Electroporation of Human Myeloid Cells (U937 and HL-60)
10. Electrotransformation of BMH 81-17mut S for Isolating Site-Directed dsDNA Mutants
11. Hanahan Method of Transformation for High Efficiency Competent Cells
12. Hanahan Transformation of E. coli
13. High Efficiency Transformation of Yeast Cells
14. High Efficiency Yeast Transformation Requiring Maintenance of a Previously Introduced Plasmid for Two-Hybrid Screens
15. High-Efficiency Electro-Transformation of E. coli
16. Infection of Cells with Retroviruses
17. Infection of Chicken Secondary Tissue Culture Cells
18. Inoue Super Competent E. coli Preparation
19. Lipofection of PC12 Cells
20. Lithium Acetate Yeast Cell Transformation Using a Heat Shock Step
21. PEG Precipitation for Making Transforming Quality DNA For Mammalian Cells
22. PEG/DMSO Production of Competent E. coli
23. Preparation of Competent E. coli Cells using Calcium Chloride
24. Preparation of Competent Yeast Cells with Lithium Acetate for Transformation
25. Preparation of Electrocompetent Cells and Electro-Transformation of E. coli
26. Preparation of Electroporation-Competent Bacteria (Strain E. coli TG1) for Phage Antibody Libraries
27. Preparation of Single-Stranded DNA for Site-Directed Mutagenesis
28. Preparing and Using Competent Bacterial Cells for Electroporation
29. Preparing Competent Yeast Cells for Frozen Storage and for Transformation
30. Procedure for Transient or Stable Transfection of Adherent Cells using Lipofectamine
31. Rapid and Simple Method of Yeast Cell Transformation
32. Rapid Yeast Transformation
33. Simple Plate Transformation of Yeast
34. Transformation of Arabidopsis thalina by Vacuum Infiltration
35. Transformation of Competent Yeast Prepared with Lithium Acetate
36. Transformation of SK1 Yeast by Electroporation
37. Transformation of Yeast Cells Using Lithium Acetate
38. Transient Transfection and Spin Infection Protocol
39. Transient Transfection of COS Cells
40. Ultra Simple Yeast Transformation